The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila.
نویسندگان
چکیده
The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45 degrees C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis sigmaA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 66 5 شماره
صفحات -
تاریخ انتشار 2002